Sentara Healthcare is a not-for-profit healthcare organization serving Virginia and northeastern North Carolina. It is based in Norfolk, Virginia, and offers services to over two million residents in Virginia and North Carolina.

Sentara operates more than 100 sites, including twelve acute care hospitals with a total of 1,911 beds, nine outpatient care campuses, seven nursing centers, three assisted living centers, and about 380 primary care and multi-specialty physicians. Sentara also provides health coverage plans, home health and hospice services, physical therapy and rehabilitation services, and specialized services, including Nightingale which, in 1982, became the region's first air ambulance.